Determinationof Caulobacter Crescentus Stalk Function throughHyphenatedProteomics Techniques
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Stalk formation of Brevundimonas and how it compares to Caulobacter crescentus
The Caulobacter crescentus cell extension known as a stalk represents an unusual bacterial morphology. C. crescentus produces stalks under multiple nutrient conditions, but the length of the stalk is increased in response to phosphate starvation. However, the exact function of the stalk is not known, nor is it known how much stalk biogenesis or function is conserved with other stalked bacteria....
متن کاملPhysiological role of stalk lengthening in Caulobacter crescentus
The Gram-negative bacterium Caulobacter crescentus forms a thin polar stalk, which mediates its attachment to solid surfaces. Whereas stalks remain short (1 µm) in nutrient-rich conditions, they lengthen dramatically (up to 30 µm) upon phosphate starvation. A long-standing hypothesis is that the Caulobacter stalk functions as a nutrient scavenging "antenna" that facilitates phosphate uptake and...
متن کاملPhospholipid biosynthesis is required for stalk elongation in Caulobacter crescentus.
Membrane phospholipid synthesis was inhibited in Caulobacter crescentus by growth of a glycerol auxotroph in the absence of glycerol or by treatment with the antibiotic cerulenin. It was observed that the final step in the swarmer cell-to-stalked cell transition, stalk elongation, was inhibited under these conditions. Since an early effect of inhibiting phospholipid synthesis in C. crescentus i...
متن کاملCaulobacter crescentus mutants with short stalks.
Limitation of inorganic phosphate in the culture medium allows stalk elongation in wild-type Caulobacter crescentus. Mutants unable to form long stalks were isolated.
متن کاملRequirement of a cell division step for stalk formation in Caulobacter crescentus.
Penicillin G at low concentrations blocked cell division in Caulobacter crescentus without inhibiting cell growth. The long filamentous cells formed after two to three generations under these conditions had a stalk at one pole and usually one or more flagella at the opposite pole. The failure of the filaments to form a second stalk at the flagellated pole indicates that stalk formation was depe...
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